Liquid Culture Syringe Instructions

How to get started with a Liquid Culture Syringe

This guide provides clear step-by-step instructions for correctly using a
liquid culture syringe for substrate inoculation. It covers the essential preparation, inspection and injection steps needed for a clean and successful inoculation process.

You’ll find practical guidance on sterile working conditions, syringe preparation and proper injection techniques to help reduce the risk of contamination and support healthy mycelium development.

Follow each step carefully and work as cleanly as possible to promote consistent, reliable colonization of the substrate.

Download manual Instruction video
Liquid culture syringe injection in sterilized substrate
Product information

Liquid Culture Syringe

Product

Liquid Culture Syringe

Description

A syringe containing 20 ml of liquid nutrients and active mycelium, suitable for direct inoculation into substrates.

Recommended for: Intermediate-level customers with prior growing experience.
What’s included

Components included

20 ml Liquid Culture Syringe

Filled with premium selected mycelium in liquid nutrients.

Sterile needle

Included for attaching to the syringe before inoculation.

Alcohol swabs

Included for disinfecting the syringe tip, needle and injection port.

1

Preparation and inspection

Before using the liquid culture syringe, inspect it carefully.

Inspect the liquid culture

Check that the liquid is clear and contains visible mycelium.

Look carefully for unusual discoloration or other visible signs that may indicate contamination.

Prepare your materials

Have the liquid culture syringe, sterile needle, alcohol swabs and chosen substrate ready before you begin.

Important: Do not use the syringe if you notice unusual discoloration or suspect contamination.
2

Prepare and disinfect the syringe

  • Remove the protective cap from the syringe.
  • Disinfect the syringe tip using an alcohol swab.
  • Carefully remove the sterile needle from its packaging.
  • Securely attach the needle to the syringe.
  • Disinfect the needle before continuing.
Keep everything clean. Avoid touching the syringe tip, needle or other sterile contact points unnecessarily.
3

Disinfect the substrate injection port

Thoroughly disinfect the injection port of the chosen substrate before inserting the needle.

Make sure the injection area is clean before proceeding.

Careful disinfection helps reduce the risk of contamination during inoculation.
4

Inject the Liquid Culture into the substrate

Gently shake the liquid culture syringe to evenly distribute the mycelium.

Insert the needle through the injection port and inject the full contents of the 20 ml syringe into the grow box or grow bag.

Use the full syringe: inject all 20 ml of liquid culture into the chosen substrate.
5

Distribute the Liquid Culture

After injection, distribute the liquid culture as evenly as possible throughout the substrate.

Grow box

Tilt the grow box sideways and gently move it up and down to distribute the liquid culture throughout the substrate.

Grow bag

Gently massage the grow bag to distribute the liquid culture throughout the substrate.

The goal is to spread the liquid culture evenly throughout the substrate.
Quick reference

Liquid Culture Syringe instructions at a glance

1. Inspect

Check the syringe and liquid culture before use.

2. Disinfect

Clean the syringe tip, needle and substrate injection port.

3. Inject

Gently mix the syringe and inject the full 20 ml into the substrate.

4. Grow box

Tilt the box sideways and up and down to distribute the culture.

5. Grow bag

Gently massage the bag to distribute the culture throughout the substrate.